毕赤酵母分泌表达华根霉脂肪酶（r27RCLC）的糖基化鉴定

Glycosylation Identification of Rhizopus chinensis Lipase (r27RCLC) Expressed in Pichia pastoris

摘要:

研究了毕赤酵母GS115中能够高效分泌表达的华根霉脂肪酶（r27RCLC）的糖基化情况。运用糖基化抑制剂衣霉素处理表达目的蛋白的毕赤酵母细胞, 通过SDS-PAGE比较分析经衣霉素处理后的脂肪酶r27RCLC分泌表达量的变化, 结果显示酶的分泌表达受到很大的抑制, 说明脂肪酶r27RCLC在表达时候很可能发生了糖基化, 且糖基化对其有重要影响。通过生物化学方法, 利用内切糖苷酶PNGaseF和EndoHf酶切处理脂肪酶r27RCLC, SDS-PAGE和Western Blot, 结果显示r27RCLC未

Abstract:

In this study, we identified the glycosylation situation of Rhizopus chinensis lipase CCTCC M201021 expressed in P. pastoris. At first, the yeast cells expressing lipase r27RCLC were treated with inhibitor tunicamycin and SDS-PAGE was conducted to analyze