卵白蛋白水解产物的抗菌性及抗氧化性

Antimicrobial and Antioxidant Activities of Ovalbumin Hydrolyzate

摘要:

以卵白蛋白为原料,采用胃蛋白酶和胰蛋白酶复合水解,对其水解产物进行体外抑菌试验,并利用A咬KTA蛋白纯化系统、透析及RP-HPLC分离得到2个具有抗菌活性的组分F21和F22。考察了2种组分对大肠杆菌细胞膜渗透性和细胞膜完整性的影响,及其DPPH·自由基清除能力。50μg/mL的F21和F22组分对大肠杆菌分别具有84.6%和22.5%的抑菌率,对金黄色葡萄球菌分别具有53.3%和29.6%的抑菌率。半乳糖苷酶释放法和流式细胞仪分析结果表明,2种产物均能破坏大肠杆菌细胞膜渗透性和细胞膜完整性。此外,F21和F22组分均具有抗氧化能力,能有效清除DPPH·自由基,当质量浓度为2.0 mg/mL时,两者DPPH·清除率接近于1.0mg/mL GSH的DPPH·清除效果。卵白蛋白水解产物可作为抗菌剂和抗氧化剂加以深度开发利用。

Abstract:

Ovalbumin hydrolyzate was prepared by pepsin and trypsin hydrolysis. Two hydrolyzate fractions with antibacterial activity F21 and F22,were separated by A咬KTA protein purification systems,dialysis,and RP-HPLC. The effects of F21 and F22 on cell membrane permeability and membrane integrity and their DPPH o radical scavenging ability were also investigated. The inhibition rates of 50 μg/mL of F21 and F22 on Escherichia coli were 84.6% and 22.5%respectively,and the inhibition rates on Staphylococcus aureus were 53.3% and 29.6%.Galactosidase release and flow cytometry analysis showed that the two fractions could destroy Escherichia coli cell membrane permeability and membrane integrity. In addition,they could effectively scavenge DPPH o radical. When the concentrations were 2.0 mg/mL,the DPPH o radical scavenging abilities of the two fractions were close to the scavenging ability of 1.0 mg/mL GSH. As a result,ovalbumin hydrolysate could be used as antibacterial agents and antioxidants in further exploitation.