坛紫菜游离氨基酸的检测分析及一种未知成分的结构鉴定

Identification of One Unknown Component and Detection Analysis of Free Amino Acids in Porphyra haitanensis

摘要:

为了检测分析坛子菜中的游离氨基酸,先采用离子交换树脂层析法分离坛紫菜粗提液中的游离氨基酸,收集3 mol/L的氨水洗脱液,分别用薄层层析、离子色谱及PITC(异硫氰酸苯酯)-HPLC柱前衍生反相高效液相色谱法对其进行检测分析。结果显示,除含有多种组成蛋白质的氨基酸外还,含有5种未知成分。采用半制备高效液相色谱系统制备分离了其中一种未知成分的衍生物TZC,经真空冷冻干燥后为白色絮状固体,结合核磁共振波谱、高分辨质谱、红外光谱数据,最终鉴定TZC去掉已知取代基团PITC后的成分为乙醇胺,分子式为C2H7NO,相对分子质量为61.08。

Abstract:

Free amino acids were separated by ion-exchange column chromatography from the crude extracts of Porphyra haitanensis. Amino acids were eluted with 3 mol/L NH3 ·3H2 O and the eluate was collected. Many protein amino acids and five unknown components were discovered in Porphyra haitanensis by thin layer chromatography,ion chromatography and PITC-HPLC respectively. One unknown derivative component(TZC) was obtained by preparation HPLC. TZC was white flocculent solid after vacuum freeze-drying. According to analysis of NMR,HR-MS,IR spectroscopy data,the molecular structure of TZC had been elucidated. TZC was ethanolamine after removed substituent component of PITC,its molecular formula was C2 H7 NO,molecular weight was 61.08.