酒酒球菌31MBR的β-D-葡萄糖苷酶活性

Assessment of β-D-Glucosidase Activity of Oenococcus oeni 31MBR

摘要:

以对-硝苯-β-葡萄糖苷为底物,采用分光光度法对酒酒球菌31MBR的β-D-葡萄糖苷酶活性进行了初步研究。结果表明:酒酒球菌31MBR具有很高的β-D-葡萄糖苷酶活性,该酶为胞内酶,且主要为可溶性酶,酶活在对数生长中期最高且随着生长时间的延长呈下降趋势。β-D-葡萄糖苷酶为结构酶,但在纤维二糖和熊果苷的诱导下酶活均有不同程度的提高。酒酒球菌31MBR具有β-D-葡萄糖苷酶活性对增加葡萄酒香气和提高葡萄酒品质具有重要意义。

Abstract:

β-D-glucosidase activity of Oenococcus oeni 31MBR was assayed spectrophotometrically with synthetic substrate p-nitrophenyl β-D-glucopyranoside.Results showed that Oenococcus oeni 31MBR exhibited considerable β-D-glucosidase activity,which was mainly intracellular form and soluble enzyme.Enzyme activity was highest at the mid-log phase,and showed a downtrend after this period.β-D-glucosidase of Oenococcus oeni 31MBR was constitutive,however,increased enzyme activities were observed when induced with both arbutin and cellobiose.Oenococcus oeni 31MBR proved potential for aroma enhancement during malolactic fermentation,this is significant for winemaking.