产β-1,3-1,4-葡聚糖酶特基拉芽孢杆菌Bacillus tequilensis CGX5-1发酵培养基的优化

Medium Optimization for the Production of β-1,3-1,4-Glucanase by Bacillus tequilensis CGX5-1

摘要:

采用响应面优化法对一株野生特基拉芽孢杆菌的发酵培养基进行优化,最终培养基各组分为:大麦粉68.4 g/L,玉米粉40 g/L,豆饼粉61.1 g/L,KH2PO41 g/L,MgSO4·7H2O 0.1 g/L,CaCl20.1 g/L。用优化培养基在37℃摇瓶发酵52 h,β-1,3-1,4-葡聚糖酶酶活达到191.96 U/mL,是优化前产酶水平的1.91倍。

Abstract:

Bacillus tequilensis CGX5-1 is a newly screened strain which can effectively secrete β1,3-1,4-glucanase.Fractional factorial design was used to clarify the medium components and the concentration of barley flour and soy bean flour were found to be the key factors.The steepest ascent experiments was applied to determine the optimal domain and the central composite design was used to estimate the quadratic response surface,thus we acquire the factor values for maximum production of β-1,3-1,4-glucanase.The final composition of fermentation medium was determined via response surface methodology,which was(g/L):barley flour 68.4,corn flour 40,soybean flour 61.1,KH2 PO4 1,MgSO4 ·7H2 O 0.1,CaCl2 0.1.The highest activity of β-1,3-1,4-glucanase was 191.96 U/mL at 52 h culture using optimized medium,which was 1.91 times higher than that from original medium.