大豆皂苷对四氯化碳致肝损伤小鼠肝脏氧化应激的干预作用

Intervention Effect of Soyasaponins on Hepatic Oxidative Stress of Mice with Liver Injury Induced by Carbon Tetrachloride

摘要:

研究大豆皂苷对四氯化碳(CCl4)所致急性肝损伤小鼠肝脏氧化应激的干预作用。将实验小鼠按体重随机分为5组,即正常对照组、模型对照组、水飞蓟素组(阳性对照组)及大豆皂苷高、低剂量组。每日给药1次,连续7 d。实验末期,除正常对照组外,其余组小鼠腹腔注射CCl4建立急性肝损伤模型,比色法检测血清白蛋白(Alb)含量、血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)活性以及肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)活性及还原型谷胱甘肽(GSH)、丙二醛(MDA)含量。结果显示,大豆皂苷明显增高CCl4致急性肝损伤小鼠血清Alb水平,降低血清ALT、AST和ALP活性;升高肝组织总SOD、CAT和GPx活性,提高肝GSH水平,降低肝MDA水平;升高肝线粒体Na+-K+-ATPase、Ca2+-Mg2+-ATPase和Mn-SOD活性,降低肝线粒体MDA含量。这说明大豆皂苷可降低CCl4致急性肝损伤小鼠肝脏氧化应激,对肝损伤具有保护作用。

Abstract:

The objective of the study was to investigate the intervention effect of soyasaponins on the oxidative stress of liver in mice with an acute liver injury induced by carbon tetrachloride(CCl4).The mice were randomly assigned to 4 groups:normal control,model control,silymarin(positive control),as well as high and low dose groups of soyasaponins.Animals were treated once daily for 7 days.At the end of the experiment,CCl4 was given intraperitoneally to the mice of groups,then the albumin(Alb),alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GPx),Na+-K+-ATPase,Ca2 +-Mg2 +-ATPase,as well as the contents of reduced glutathione(GSH) and malondialdehyde(MDA) were detected by the colorimetric method.The results showed that the administration with soyasaponins increased the serum Alb level and reduced the ALT,AST and ALP activities of serum,increased the total SOD,CAT,GPx and GSH level and decreased the MDA content of liver,increased the Na+-K+-ATPase,Ca2 +-Mg2 +-ATPase and Mn-SOD activities and decreased the MDA content of liver mitochondria in mice with acute liver injury.It is suggested that the soyasaponins reduces the oxidative stress of liver tissue in mice with acute liver injury induced by CCl4 and has a protective effect on liver injury.