三种加工方式海带中砷形态和含量的比较

Comparison of Three Kinds of Processing Methods on Arsenic Speciation and Content in Laminaria japonica

摘要:

以不同加工方式(直接干燥、漂烫、漂烫-盐渍-水洗)的海带为研究对象,采用高效液相色谱-电感耦合等离子体质谱(HPLC-ICP-MS)联用技术测定砷的形态和含量。结果表明,直接干燥海带和漂烫后海带总砷含量分别为47.23 mg/kg和42.91 mg/kg(以干重计),无显著差异(P>0.05);漂烫-盐渍-水洗后的海带总砷含量为25.62 mg/kg(以干重计),与直接干燥海带和漂烫后海带相比差异极显著(P<0.01);海带中总砷含量经漂烫-盐渍-水洗后逐步减少,尤以盐渍水洗过程减少最明显。海带中砷的主要形态为有机态,分别是砷胆碱、砷甜菜碱、二甲基砷和3种疑似砷糖的物质,其中砷糖质量分数最高(w>95%),无机砷质量分数最低(w<1%)。

Abstract:

The aims of this manuscript were to study arsenic speciation and content in kelp with different processing methods(direct drying,blanching,blanching-salting-washing) by high performance liquid chromatography coupled to inductively coupled plasma-mass spectrometry(HPLC-ICP-MS).The results showed that the total arsenic content in direct drying kelp was 47.23 mg/kg and blanching kelp was 42.91 mg/kg(dry weight) with no significant differences(P>0.05).The total arsenic content in blanching-salting-washing kelp was 25.62 mg/kg(dry weight),there was extremely significant differences(P<0.01) from direct drying kelp and blanching kelp.The total arsenic content in kelps was gradually decreased after blanching-salting-washing,while the saltingwashing process decreased most obviously.Arsenocholine,arsenobetaine,dimethylarsinic acid and three substances suspected as arsenicsugar were the major arsenic forms in kelps,which arsenicsugar was in the hightest amount(w>95%) and inorganic arsenic was in the lowest amount(w<1%).