酶联免疫法检测食品中乳酸链球菌素

Method of Nisin Detection in Food by Enzyme-Linked Immunosorbent Assay

摘要:

建立了酶联免疫吸附法来检测乳制品、肉制品和含乳饮料中的乳酸链球菌素含量。在抗乳酸链球菌素多克隆抗体的基础上,建立间接竞争酶联免疫吸附法。所建立的间接竞争ELISA方法的灵敏度为0.1μg/mL,标准曲线范围为0.1～62.5μg/mL,乳制品的检测限为1.0μg/mL,肉制品的检测限为0.5μg/g,样本的添加回收率范围在80%～120%之间,变异系数<20%。所建立的间接竞争ELISA方法及制备的相关试剂盒具备准确、快速、简便的优点,能够满足乳制品、肉制品和含乳饮料中乳酸链球菌素含量的检测要求。

Abstract:

The aim of this study was to develop a rapid quantitative melamine ELISA test kit for the detection of Nisin in dairy,meat and milk drink.The indirect competitive enzyme-linked immunosorbent assay was established based on the Nisin specific polyclonal antibody.The sensitivity was 0.1 μg/mL,the standard curve range was 0.1～62.5 μg/mL,the detection limit of the dairy and meat were 1.0 μg/mL,and 0.5 μg/g,respectively.The average recoveries of milk drink and meat ranges between 80% ～120%,and the coefficient of variation was <20%.The indirect competitive ELISA has been proven to be rapid,sensitive,and simple.It is suitable for the detection of the Nisin content in dairy,meat and milk drink.