草苁蓉水萃取物对脂多糖与D-氨基半乳糖所致小鼠肝脏氧化应激和细胞凋亡的影响

Effect of Aqueous Fraction of Boschniakia rossica on Hepatic Oxidative Stress and Hepatocyte Apoptosis of Mice with Acute Liver Injury Induced by Lipopolysaccharide and D-Galactosamine

摘要:

研究草苁蓉水萃取物(BRAF)对脂多糖(LPS)与D-氨基半乳糖(GalN)联合诱发的小鼠急性肝损伤的保护作用。将小鼠按体质量随机分为5组,即正常组、模型组,BRAF高、低剂量组及水飞蓟素(SIL)组(阳性对照组)。每日给药1次,连续7 d。实验末期,除正常组外,其余组小鼠腹腔注射LPS和GalN建立急性肝损伤模型,苏木素-伊红(HE)染色法观察肝组织病理学变化,ELISA法检测血清肿瘤坏死因子-α(TNF-α),比色法检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)以及肝脏过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽-S-转移酶(GST)活性及还原型谷胱甘肽(GSH)、丙二醛(MDA)和一氧化氮(NO)含量,电泳法检测小鼠肝细胞DNA损伤情况。结果显示,BRAF明显降低GalN和LPS联合诱导的急性肝损伤小鼠血清ALT、AST活性,减轻肝组织病理损伤,降低血清TNF-α水平,降低肝NO和MDA水平,升高肝组织CAT、GPx、GST活性和GSH水平,并降低肝细胞DNA损伤程度。提示:BRAF对GalN和LPS联合诱导的小鼠急性肝损伤具有保护作用,其机制可能与其抗氧化活性和抗肝细胞凋亡作用有关。

Abstract:

Effect of the aqueous fraction from ethanolic extract of Boschniakia rossica(BRAF) on the oxidative stress and apoptosis of liver in mice with an acute liver injury induced by Lipopolysaccharide(LPS) and D-galactosamine(GalN) was investigated in this manuscript.The mice were randomly assigned to 5 groups: normal control,model control,silymarin(positive control),as well as high and low dose groups of BRAF.Animals were treated once daily for 7 days.At the end of the experiment,LPS and GalN were given intraperitoneally to the mice of groups,then the pathological changes was detected with hematoxylin & eosin(HE) staining,tumor necrosis factor-α(TNF-α) was detected by ELISA method,the alanine aminotransferase(ALT),aspartate aminotransferase(AST),catalase(CAT),glutathione peroxidase(GPx),glutathione S-transferase(GST),reduced glutathione(GSH),malondialdehyde(MDA) and nitric oxide(NO) were assayed with colorimetric method,and DNA damage was analyzed using the electrophoretic method.The results showed that the administration with BRAF reduced the serum ALT and AST,alleviated the hepatic lesions induced by GalN/LPS,decreased serum TNF-α level,reduced the hepatic NO and MDA contents,increased the hepatic CAT,GPx,GST and GSH levels,and reduced the DNA damage of hepatocyte in mice with acute liver injury.It is suggested that BRAF has a protective effect on acute liver injury induced by GalN and LPS in mice,probably via antioxidative and anti-apoptotic abilities.