芦笋醇提取物的免疫调节作用

Studies on Immune Regulation Effect of Ethanol Extract from Asparagus

摘要:

分析芦笋醇提取物对机体免疫功能的调节作用。将风干芦笋用75%体积分数的乙醇溶液进行超声提取,获得芦笋醇提取物。对实验小鼠灌胃芦笋醇提取物,剂量分别设为7、70、140 mg/kg,另设一个空白对照组灌胃等体积生理盐水,连续30 d灌胃后,分别进行小鼠脏器/体重、腹腔巨噬细胞吞噬实验、淋巴细胞转化实验及半数溶血值实验。结果显示,中、高剂量组的芦笋提取物可显著提高小鼠的脾脏指数和腹腔巨噬细胞的吞噬能力;中、高剂量组的芦笋提取物具有显著增强淋巴细胞增值能力的作用,对提高血清溶血素含量作用极显著,并具有剂量依赖性。表明芦笋提取物能增强正常小鼠的非特异性和特异性免疫功能。

Abstract:

In this study,air dried asparagus was ultrasonic extracted with 75 %(v/v)ethanol.The immune function of this extract on mice were investigated.Mice were orally administrated with asparagus extract at three dose levels(7,70,140 mg/Kg BW) for 30 days.At the organ level,thymus and spleen were weighed and their indices were calculated.The macrophage phagocytosis ability in vitro,transformation of spleen lyphmocytes,and serum hemolysin were investigated to assess the immune functions.The results showed that the middle and high dosage of asparagus extract promoted the spleen index and the phagocytic function of murine peritoneal macrophages.the middle and high dosage of asparagus extract significantly enhanced the transformation of spleen lyphmocytes and serum hemolysin(HC50).This suggest that asparagus extract can enhance both the specific and non-specific immune functions in normal mice.Moreover,the specific immune effects of asparagus extract especially depended on the dosage.