脂肪酶抑制剂产生菌的筛选和鉴定

Screening and Identification of Lipase Inhibitor-Producing Strain

摘要:

通过对不同地点采集样品,分离纯化得到的不同单菌落,再进行发酵培养,并以其产物是否对脂肪酶具有抑制活性作为筛选指标,从2013株野生菌中筛选到一株产脂肪酶抑制活性较高的菌株Sf16。对菌株Sf16的菌落、气生菌丝、孢子丝及孢子的形态特征和在不同碳源培养基上的生长表现的特征以及一些生理生化方面特性进行观察,并对菌株的DNA进行提取并扩增16SrDNA,通过对后者序列测定、数据库同源搜索和比对,进而构建菌株的系统进化树。通过形态学和分子生物学手段对产脂肪酶抑制剂菌株Sf16进行初步鉴定,结果确定其为Streptomyces sp.。

Abstract:

We collected many samples from different locations,then isolated and purified single colony. By fermentation,the products whether have lipase inhibition activity as a screening indicator. We find a good strain Sf16 after we cultured 2013 wild strains. The morphological characteristics of colony,aerial hyphae,sporothrix and spores and the growth performance characteristics in different carbon sources medium of strain Sf16,as well as some physiological and biochemical characteristics were observed. Extract the DNA,amplified and determined the 16SrDNA.Then homology search in database,compared and constructed the phylogenetic tree. Inhibitor-producing strain Sf16 preliminary identification by means of morphological and molecular biology techniques,the result is Streptomyces sp.