维生素对竹黄菌发酵产苝醌类色素的影响

Effect of Vitamins on Perylenequinones Production of Shiraia bambusicola

摘要:

采用单因素法研究了生物素、VB1、VB3、VB5、VB6,肌醇对氨基苯甲酸(PABA)和VC对竹黄菌Shiraia sp.SUPER-H168生长及其次级代谢产物苝醌类色素积累的影响。结果表明,添加VB5和VB1可显著促进竹黄菌的生长和色素的积累,菌体量和色素产量分别较对照提高了37.8%和31%,VB5和VB1的较适质量浓度为0.5 mg/L和1.0 mg/L。添加生物素和对氨基苯甲酸可显著提高菌体生长量,但低质量浓度的添加量对色素的积累起抑制作用。添加VB3、肌醇和VC可略微提高色素的产量,较适质量浓度为0.5、0.05、0.05 mg/L。添加VB6虽然可促进菌体的生长,但却抑制了色素的积累。将对色素积累有促进作用的VB3、VB5、肌醇、VB1、VC这5种维生素最优质量浓度下混合添加到5 L发酵罐培养基中,观察了菌体生长和发酵产色素的过程,最终的菌体量和色素产量较对照分别提高了33.7%和75%。

Abstract:

The effect of biotin,VB 1 ,VB 3 ,VB 5 ,VB 6 ,inositol,para aminobenzoic acid(PABA) and VC on the growth of shiraia sp.SUPER-H168 and perylenequinones production were studied with single factor experiment. The results showed that biotin and PABA strongly improved the biomass of cell, but low concentration of them could inhibit the pigment production. VB 5 and VB 1 slightly increased the production of pigment,and their optimum concentration were 0.5,0.05,0.05 mg/L. Although VB 6 could improve the growth of fungi,the synthesis of the pigments was inhibited. The mixed vitamins containing VB 1 ,VB 3 ,inositol,VB 5 and VC was added into 5L -fermentation medium with the optimum concentration because of their improvement of pigment yield,and their effect on the growth of shiraia sp.SUPER -H168 and pigment yield was studied. The final biomass and pigment yield were increased by 33.7% and 75% respectively compared to the experiment without vitamin.