不同碳源对黑芝菌丝体液体发酵的影响

Effects of Different Carbon Sources on Mycelium Submerged Fermentation of Ganoderma atrum

摘要:

对黑芝菌丝体液体发酵体系进行了研究。实验中以黑芝菌丝体生物量为主要评价指标,通过正交实验研究了无机盐、维生素和碳源在黑芝菌丝体发酵中的交互作用。结果显示:黑芝菌丝体对果糖的吸收利用要优于蔗糖和乳糖,且当碳源种类不同时,黑芝菌丝体对无机盐和维生素的需求也明显不同。实验中筛选出的较适宜黑芝菌丝体液体发酵的培养基为:果糖20 g/L+KH2PO42 g/L+K2HPO42 g/L+MgSO4.7H2O 2 g/L+CaCl2.2H2O 1 g/L+VB120 mg/L,pH自然。黑芝菌丝体在此培养基中发酵培养7 d,菌丝生物量可达0.808 6 g/dL。

Abstract:

In this manuscript,the effect of carbon sources on the mycelium growth of Ganoderma atrum in submerged fermentation was studied by orthogonal experiment.furthermore,the the interactions of inorganic salt,vitamin and carbon source were also discussed.The main results was obtained and listed as follows:1) fructose was much better than that of sucrose and lactose on mycelium reproduction,and the demands for inorganic salt and vitamin were dependent on the kinds of carbon sources;2) the optimum medium for mycelium was 20 g/L fructose,2 g/L KH2PO4,2 g/L K2HPO4,2 g/L MgSO4·7H2O,1 g/L CaCl2·2H2O,20 mg/L VB1,natural pH.with the optimum medium,the the mycelium biomass of G.atrum could reach at 0.808 6 g/dL after 7 days culture.