SYBR Green实时荧光PCR检测食品中鲨鱼源性成分真实性方法的建立

Authentication of Shark Derived Material in Food Using SYBR Green Fluorescence Real-Time PCR

摘要:

为确保鲨鱼产品的真实性,作者研究建立了食品中鲨鱼源性成分的SYBR Green实时荧光PCR鉴定方法。运用建立的SYBR Green实时荧光PCR方法对9种鲨鱼及48种常见非鲨鱼类动植物样品进行检测,9种鲨鱼样品均出现扩增曲线,且熔解曲线在(84±1.5)℃内出现峰值;其他非鲨鱼样品中均未出现扩增曲线,熔解曲线在(84±1.5)℃也未出现峰值。该检测方法的检测限为0.000 1 ng/μL鲨鱼DNA和质量分数0.01%狗鲨肉粉。运用建立的方法对20种常见的鲨鱼产品进行PCR检测,除仿鱼翅和鲨鱼肝油外所有产品中均能检测出鲨鱼成分。结果表明,该检测方法特异性强,灵敏度高,能够用于食品中鲨鱼源性成分的真实性鉴别。

Abstract:

A specific and sensitive SYBR Green real-time fluorescence PCR method was developed to determine of the shark derived material in food.The specificity and sentivity of this method was examined.The results showed 9 kinds of shark samples displayed theamplified curves and the highest point of melt curves at(84±1.5) ℃,whereas other 48 kinds of common plants and animals displayed no amplified curves and the highest point of melt curves at temperatures out of(84±1.5) ℃.The detection limit of the method was 0.000 1 ng/μL DNA and 0.01% shark meat powder.The 20 commercial shark products samples were analysized by SYBR Green PCR and all the products indicated existence of shark DNA except the artifical shark fin and shark liver oil.The results indicated that the detection method is highly specific and sensitive,and can be used to the identification of shark derived material in food.