生物柴油中甾醇糖苷的富集、纯化及含量测定

Enrichment,Purification and Determination of Sterol Glycosides in Biodiesel

摘要:

研究了生物柴油中甾醇糖苷(Sterol Glycosides,SG)富集,纯化及气相色谱定量测定的方法。通过皂化,液液萃取及固相萃取(SPE),富集和纯化甾醇糖苷,采用β-胆甾烷醇作为内标,对其进行气相分析并定量。讨论了生物柴油及其副产物乳化生物柴油和生物柴油残渣的不同前处理,考察了皂化强度对甾醇糖苷含量测定的影响。结果表明:生物柴油样品不需皂化,生物柴油副产物乳化生物柴油和生物柴油残渣皂化条件为:皂化溶液:0.1mol/L KOH-乙醇,皂化温度为60℃,皂化时间:40min。

Abstract:

The method for enrichment,purification and determination of glycosides in biodiesel by GC was developed in this manuscript.Sterol glycosides was purified and enriched by saponification,liquid-liquid extraction,SPE(Solid Phase Extraction),and quantified using β-cholestanol as internal standard by GC.The different pre-treatments methods for biodiesel,emulsified biodiesel and biodiesel residue and effect of strength of saponification on determination of sterol glycosides were discussed.The results showed that: biodiesel was treated without saponification,and the optimum conditions for emulsified biodiesel and biodiesel residue listed as follows: that t 0.1mol/L KOH-ethanol solution,60℃saponifying temperature,40min saponifying time.The results suggested that were helpful to improve the control of biodiesel quality and the utilization of by-products in biodiesel.