猪源降胆固醇乳酸菌的分离鉴定

Isolation and Identification of Cholesterol-Reducing Lactic Acid Bacteria from Pig

摘要:

乳酸菌是一种重要的益生菌,具有降解胆固醇能力。作者从猪消化道中分离出31株疑似菌株,在高胆固醇培养基中培养后有18株菌株生长。利用磷硫铁法对体外降胆固醇进行了研究,结果表明,胆固醇降解率在0～47.26%,其中4株乳酸菌的胆固醇降解率大于30%,分别是r16、r53、r762和m661。通过细胞形态、生理生化试验和16SrDNA分子生物学鉴定,最终鉴定r16、r53和r762为植物乳杆菌,m661为鹑鸡肠球菌。

Abstract:

Lactic acid bacteria are a kind of important probiotics,which have the ability of cholesterol-reducing.Total 31 strains of Lactic acid bacteria were isolated from pig source.Among these strains,18 strains could grow in the medium containing high cholesterol using phosphate pyrite method and the cholesterol-removal rate of those strain varified from 0 to 47.26% in vitro.Among them,four strains,namely r16,r53,r762,and m66,exhibit high cholesterol reducing ability more than 30%,.Based on the morphological,physiological,biochemistry characteristics and 16S rDNA sequencing analysis,strain r16,r53,and r762 were identified as Lactobacillus plantarum,and m661 as Enterococcus gallinarum.