奥曲肽对高脂饲养小鼠血糖、血脂及抗氧化能力的影响

Effects of OCT on Blood Glucose、Lipid and Anti-Oxidation Function of Mice Fed with High-Fat Diet

摘要:

研究奥曲肽(OCT)对高脂饲养小鼠血糖、血脂及抗氧化能力的影响。正常组(control)、高脂组(HFD)、高脂+奥曲肽组(OCT)、高脂+质量分数0.1%硫辛酸(LA)组雄性C57BL/6小鼠喂养3周,在第3周期间腹腔注射奥曲肽0.1mL(20μg/kg),3周后进行葡萄糖耐量试验,测定血TC、TG、HDL-C、LDL-C、AI水平,肝脏、肌肉、脂肪、脑组织ROS、CAT、T-AOC、MDA水平。结果表明:注射OCT能够显著降低高脂饲养小鼠葡萄糖耐量试验血糖曲线下面积及TC、TG、LDL-C、AI水平(p<0.05),显著升高HDL-C水平(p<0.05),显著降低高脂饲养小鼠肝脏、肌肉、脂肪、脑组织ROS水平(p<0.05),显著提高CAT、T-AOC水平(p<0.05),表明注射奥曲肽可以改善高脂饲养小鼠的血脂代谢,缓解代谢组织的氧化应激状态。

Abstract:

The objective of this study is to investigate the effects of OCT on blood glucose,lipid and anti-oxidation function of mice fed with high-fat diet.For this,Process and Methods: Three weeks feeding of C57BL/6 mice was divided into normal group(control),high-fat group(HFD),high-fat +octreotide(OCT) group,high-fat +0.1 wt % lipoic acid(LA) group,and feed 3 weeks.Among them,the OCT group were injected 0.1mL of OCT(20?g/ k g) during the third week,oral glucose tolerance test(OGTT) was done at the end of the third week.The levels of blood TC,TG,HDL-C,LDL-C,AI,levels ROS,CAT,T-AOC,MDA of liver,muscle,fat and brain were measured.It was found that OCT significantly reduce the curve size of blood glucose in OGTT experiment.Compared with HFD group(p<0.05),OCT decreased the levels of TC,TG,LDL-C,AI but increase HDL-Clevel.OCT injection could significantly reduce the levels of ROS,MDA and increase the levels of CAT,T-AOC in tissues of liver,muscle,fat and brain compared with HFD group(p<0.05,which indicated that OCT injection can improve lipid metabolism and alleviate oxidative stress in mice fed with high-fat diet.