基于谷氨酰胺转胺酶催化交联的羊毛角蛋白成膜性能的研究

Study on Filming Performance of Wool Keratin with the Catalysis of Transglutaminase

摘要:

采用谷氨酰胺转胺酶(TGase)催化羊毛角蛋白发生交联反应,探讨了酶用量、成膜溶液的pH值、温度、处理时间对羊毛角蛋白膜抗拉强度及断裂伸长率的影响,并考察了酶促交联反应对羊毛角蛋白膜在水中稳定性的影响。借助电泳(SDS-PAGE)对TGase催化羊毛角蛋白交联反应引起蛋白质相对分子质量的变化进行表征。研究结果表明,TGase催化羊毛角蛋白发生交联反应提高了其成膜的抗拉强度和稳定性,降低了膜的断裂伸长率。较好的工艺条件为:酶用量30 U/g角蛋白,成膜pH值为7.5,温度40℃,时间18 h左右。SDS-PAGE结果表明,羊毛角蛋白在TGase的催化作用下发生共价交联形成了相对分子质量更大的蛋白聚合物。

Abstract:

In this study,transglutaminase was adopted to catalyze wool keratin cross-linked and the effect of reaction conditions such as enzyme dosage,pH value of film-forming solution, temperature,treatment time on the mechanical properties of the film were determined.In addition, the effect of enzymatic cross-linking reaction on the stability of the wool keratin film in hot water was investigated.The keratin protein molecular change induced by TGase-mediated cross-linking was analyzed by SDS-PAGE.The results showed that,TGase-catalyzed preparation of keratin film could increase tensile strength and stability of water,but decrease the elongation. The optimum conditions were as follows:30 U/g wool keratin of TGase,40℃,pH 7.5 of film-forming solutions,18 h of treatment time.SDS-PAGE result demonstrated that new biopolymers with higher molecular weight from wool keratin were formed by using TGase.