啤酒中脱氧雪腐镰刀菌烯醇含量的测定

Determination of Deoxynivalenol in Beer

摘要:

采用高效液相色谱法对啤酒中脱氧雪腐镰刀菌烯醇(DON)进行分析检测。选用色谱柱Zorbax Eclipose XDB-C18(4.6mm×250mm,5μm),流动相为甲醇-0.05%磷酸水溶液(体积比20∶80),柱温30℃,流速1.0mL/min,检测波长210nm。在此条件下,DON得到很好分离,在0.75～100mg/L范围内线性关系良好,R2=0.999 3,外加标回收率在85.36%～96.92%之间,RSD为4.21%,该方法可以简便、准确地测定啤酒中DON含量。

Abstract:

An analytical method was developed to determine deoxynivalenol content in beer by high performance liquid chromatography in this study.Separations were achieved on a Zorbax Eclipose XDB-C18(4.6 mm×250 mm,5 μm)in a linear gradient,and the mobile phase was a mixture of methanol and water with phosphoric acid(0.05%,v/v)at(20︰80,v/v.In addition,the column temperature was maintained at 30 ℃ with a flow rate of 1.0 mL/min.The detection wave length was set at 210 nm.There was a good linear relationship between 0.75 mg/L-100 mg/L(R2=0.999 3).The average recovery was between 85.36%~96.92%,and RSD was 4.21%.It was proved to be a convenient and accurate method for analyzing deoxynivalenol content in beer.