高超氧化物歧化酶活性海洋红酵母的筛选

Screening of Rhodotorula marina of High Superoxide Dismutase Activity

摘要:

为了提高海洋红酵母超氧化物歧化酶的活性,寻找一种可以从众多菌株中定向筛选超氧化物歧化酶高产菌株的方法,通过在培养基中添加百草枯并逐级提高、层层筛选,筛选出了超氧化物歧化酶活性较高的菌株并研究了不同程度的氧化胁迫对海洋红酵母的生长状况、超氧化物歧化酶活性及虾青素质量分数的影响。结果表明:在百草枯浓度为0.075mmol/L的条件下,可大大提高超氧化物歧化酶活性;经逐步提高百草枯浓度所筛选的菌株超氧化物歧化酶比活性提高35.2%;而所筛菌株在含0.075mmol/L的百草枯培养基中培养后,超氧化物歧化酶比活性较初始菌株提高103.7%,差异显著(p<0.05)。这说明使用该方法可以达到筛选超氧化物歧化酶高产菌株的目的,而在培养基中添加低浓度的百草枯同样可提高海洋红酵母的超氧化物歧化酶活性。

Abstract:

To improve the superoxide dismutase activity of Rhodotorula marina,a screen method with paraquat as drug was introduced in this study.Though gradually increasing of paraquat content in medium,a stain with of high superoxide dismutase activity(increased by 35.2%) was screened.Based on this,the effect of different oxidative stress to the growth,superoxide dismutase activity and astaxanthin content was investigated.The results indicated that 0.075 mmol/L paraquat could improve the superoxide dismutase activity by 103.7% when compared with that of the control,(p<0.05).This shows that this method can achieve the purpose of screening strain with high superoxide dismutase activity,and culturing with low concentrations of paraquat can also improve the superoxide dismutase activity of Rhodotorula marina.