真姬菇JB06发酵海带废渣所得产物的抗自由基和总抗氧化能力

Anti-Free Radical and Antioxidant Activity of Exopolysaccharide from Fermented Kelp Waste by Hypsizigus marmoreus JB06

摘要:

为了探索海带废渣高附加值利用的方法,用真姬菇JB06菌株液态发酵海带废渣,乙醇沉淀法制备发酵液上清液中多糖,用试剂盒测定了所得多糖的抗自由基和总抗氧化能力。结果表明:在多糖质量浓度为0.5mg/mL时,真姬菇JB06菌株发酵上清液中的多糖(简称JB06菌株多糖)对羟自由基的抑制率比海带废渣水溶液上清中的多糖(简称对照多糖)高了11.34%;多糖质量浓度为8mg/mL时,JB06菌株多糖对超氧阴离子自由基的抑制率比对照多糖高了9.52%;JB06菌株多糖的总抗氧化能力比对照多糖高出了13.20%。通过实验可以得出:真姬菇发酵海带废渣后获得的多糖的总抗氧化能力比对照多糖总抗氧化能力显著提高。

Abstract:

The aim of this study is to find a way to reuse of kelp waste with higher value.For this,kelp waste was fermented in liquid by Hypsizigus marmoreus JB06,and the polysaccharides were got through sedimentation by ethanol.The activity of anti-free radical and total antioxidant of polysaccharides was detected by using the Kit.The results showed that at the 0.5 mg/mL,the inhibition rate of hydroxyl radical of the polysaccharide was higher by 11.34% than that from the solution of pure kelp waste.At the content of 8mg/mL,the inhibition of superoxide anion was higher by 9.52 % than that from the solution of pure kelp waste;the total antioxidant activity was increased by 13.20%.Based on the above results,it can conclued that the antioxidant capacity was significantly increased by fermentation.