辽五味子黄酮纯化及定性光谱分析

Separation Purification and Spectrum of Flavonoids from Liao Schisandra chinensis

摘要:

选用4种大孔树脂对辽五味子黄酮进行了静态吸附与解吸附试验。结果表明,HZ-818型树脂对黄酮的吸附率可达76.24%,解吸率达到67.30%,与其他4种型号树脂相比最适宜于辽五味子黄酮的分离纯化。通过对HZ-818树脂动态吸附试验,确定黄酮加样质量浓度为1.6mg/mL时,上样体积为65mL,上样流量1mL/min,洗脱流量1.5mL/min,用体积分数70%的乙醇进行洗脱,洗脱液用量为110mL,可达到较好的洗脱效果,洗脱率可达到79.57%。提取物经光谱分析,证实为黄酮类化合物。

Abstract:

This manuscript select four different macroporous resins that were used to static adsorption and desorption attached experiment for flavonoids of Liao Schisandra chinensis(Turcz.)Baill.Among them,the highest adsorption capability of 76.24% and desorption rate of 67.30% were achieved at macroporous resin HZ-818 and was chosen.By using resin HZ-818,the optimum process conditions was determined and listed as follows: sample concentration 1.6 mg/mL,the sample size 65ml,and sample flow rate 1mg/min;70% ethanol as eluent at 1.5 mL/min,and eluent dosage for 110 mL.Under the optimum conditions,the elution ratio of flavones achieved at 79.57%.