一株转化茴脑生成茴香醛的菌株筛选与鉴定

Isolation and Characterization a Strain for Bioconversion Anethole to Anisaldehyde

摘要:

以反式茴脑为惟一碳源,通过富集培养和分离纯化,从环境中筛选到320株能以反式茴脑作为惟一碳源的菌株。经薄层层析法、紫外分光光度法和气相色谱法相结合对转化液进行分析,结果获得一株相对高产茴香醛的细菌菌株WGB30。该菌最适生长温度28℃,最适pH 6.5,在复筛培养基中,30℃摇床200r/min培养12h可达到对数期,在培养60h时茴香醛的摩尔转化率达5.08%。综合形态学、生理生化实验、Biolog EcoPlates鉴定和16SrDNA序列分析结果,将菌株WGB30鉴定为伯克霍尔德氏菌(Burkholderia sp.)。

Abstract:

Using trans-anethole as the sole carbon source,a strain WGB30 was screened from 320 isolates,and 50 environmental samples by thin-layer chromatography,high efficiency liquid chromatography and spectrophotometry.It was found that the optimum temperature and pH is 28 ℃ and 6.5 for this strain,respectively.One loopful of the plate culture was inoculated into 100 mL medium containing 0.5% anethole as carbon source incubated at 30 ℃ for 60 h with reciprocal shaking(200 strocks/min),the ratio conversion of anisaldehyde is up to 5.08%.According to the morphological features,the results of physiological and biochemical experiments and 16S rDNA sequence analysis,this strain was identified as Burkholderia sp.