反式脂肪酸Trans C18∶1诱导内皮细胞凋亡的研究

Apoptosis of Human Umbilical Vein Endothelial Cells Induced by Trans C18∶1

摘要:

通过检测相关细胞凋亡指标,探讨trans C18:1诱导内皮细胞的凋亡机制。采用体外细胞培养方法将不同浓度trans C18∶1与内皮细胞共同孵育后,通过吉姆萨染色,观察内皮细胞形态变化;采用流式细胞仪检测细胞的早期凋亡变化;通过试剂盒检测观察trans C18:1对凋亡酶Caspase活力的影响;最后考察Caspase抑制剂z-VAD-fmk,对trans C18∶1对内皮细胞存活率的影响。吉姆萨染色后,细胞呈典型的凋亡形态;流失细胞仪检测内皮凋亡细胞的数量明显增加;同时发现trans C18∶1引起内皮细胞的凋亡可能与Caspase-3酶激活有关,因为Caspase抑制剂又能提高trans C18∶1处理的内皮细胞存活率。

Abstract:

The target of this study is to exoplore the apoptotic mechanism of endothelial cells through detecting relevanting apoptosis index.For this,EC were cultured with trans C18∶1 at different concentrations,then the morphological changes of endothelial cells were observed through giemsa staining.The apoptotic cells were detected by flow cytometry.The activity of caspase-3 was detected by spectrophotography after endothelial cells treated with trans C18∶1.The cell viability was measured by MTT after endothelial cells cultured with trans C18∶1 and caspase inhibitor for 24～48 h.Results: The number of apoptotic cells raised and endothelial cells displayed classical apoptotic morphology after endothelial cells cultured with trans C18∶1.The activity of caspase-3 ascended and the caspase inhibitor could protect endothelial cells from trans C18∶1.