基于发酵液特性的聚唾液酸提纯工艺的研究

Research on the Purication of Polysialic Acid Based on the Property of the Fermentation Broth

摘要:

为了从发酵液中提纯制备聚唾液酸,作者对聚唾液酸特性和发酵液中主要杂质的去除方法进行了研究。结果表明,聚唾液酸具备一定的热稳定性和抗碱性分解能力,并且在一定条件下,可以被乙醇和阳离子表面活性剂氯代十六烷基吡啶(CPC)沉淀。发酵液中的主要杂质可以在一定条件下利用以珍珠岩助滤的方式除去。最终构建了聚唾液酸提纯工艺,利用该工艺得到的产品纯度达到了(98.1±1.6)%,回收率为(56.1±1.7)%。

Abstract:

In order to obtain Polysialic acid(PSA) from the fermentation broth,the property of PSA and methods to remove the impurities in the fermentation broth were studied in this manuscript.The results demonstrated that PSA showed a good heat-stability and anti-alkalescence capability,and it was precipitated by ethanol and cetyl pyridinium chloride(CPC) in certain condition;impurities in the fermentation broth can be removed by filtration with Perlite as filter aid.Based on this,a novel PSA purification process was established.Through this process, the final PSA product was obtained with a purity and recovery(98.1±1.6)%and(56.1±1.7)%,respectively.