核酸适配体功能化金纳米探针识别-共振光散射法检测腺苷

Resonance Light Scattering Detection of Adenosine Based on Aptamer Functionalized Nanogold Probe Recognition

摘要:

将纳米探针技术、核酸适配体识别与共振光散射检测技术有机结合,以腺苷为模式分析物,通过均相体系中腺苷与其特异结合核酸适配体识别反应,使形成网络聚集结构的金纳米结构解聚,从而引起共振光散射信号强烈变化。基于此,建立了核酸适配体识别的均相检测腺苷的新方法。该方法对腺苷检测的浓度线性范围为0.1～10μmol/L,检测限为0.04μmol/L(3S/N)。该方法对腺苷的检测灵敏度高、选择性好,鸟苷、胸苷、胞苷、尿苷等对检测不产生干扰。

Abstract:

Coupling nano-probe technology,aptamer recognition with resonance light scattering analysis,through recognizing reaction between adenosine and its specific aptamer in homogeneous matrix,causing the disaggregation of the aggregated network structure of adenosine specific aptamer functionalized nanogold structure,a sharply change on the resonance light scattering signal can be obtained.Based on it,a novel homogeneous aptamer recognizing based method for adenosine detection was established.The linear concentration range was 0.1～10μmol/L for adenosine with a detection limit of 0.04μmol/L(3S/N).The proposed method also shows good selectivity for adenosine detection and no interference from guanosine,thymidine,cytidine and uridine.