乳酸乳杆菌胞外多糖的发酵条件优化

Optimization of Fermentation Conditions for Exopolysaccharid Production from Lactobacillus lactis

摘要:

对乳酸乳杆菌发酵生产胞外多糖的发酵条件进行了优化。在确定Elliker培养基为最适培养基的基础上,利用Plackett-Burman试验设计,筛选出对胞外多糖产量有显著影响的因素,即酵母粉、发酵时间、初始pH值,然后针对这3个关键因子,用单因子试验及中心组合试验优化得到最佳发酵条件。结果表明:当酵母粉为17.35 g/L,初始pH 5.85,发酵时间23.18 h时,乳酸乳杆菌胞外多糖实际产量可达3.910 g/L。

Abstract:

This study aims to increase exopolysaccharid production d by optimizing the culture and nutrient conditions of Lactobacillus lactis.The results showed that Elliker was the optimum medium.Three statistically significant ingredients,yeast extract,the initial pH and fermentation time,which were determined by Plackett-Burman design.Based on the results of the first phase,the five-level three-factor central composite design was employed to optimize the above critical factors.The exopolysaccharid titer was achieved at 3.910 g/L,with the combination of yeast extract 17.35 g/L,the initial pH 5.85,fermentation time 23.18 h.