Cosmosil Cholester-HPLC法分离番茄红素异构体的研究

Study on the Separation of Lycopene Isomers Via Cosmosil Cholester-HPLC System

摘要:

番茄红素是一种由11个共轭双键和2个非共轭双键组成的直链型多不饱和烯烃。由于分子中双键数目较多,番茄红素有很多空间异构体。对COSMOSIL Cholester-HPLC法分离番茄红素异构体的方法进行了研究并优化了分离条件。最终确定的试验条件如下:流动相为V(四氢呋喃)∶V(乙腈)=10∶90,进样量20μL,流速1 mL/min,检测波长472 nm。此试验条件下进行的重复性试验表明,该方法分离效果比较理想,重复性良好。

Abstract:

Lycopene is a ploy-unsaturated carotene with 11 conjugated and 2 non-conjugated carbon-carbon double bonds.Because of its 13 double bonds,lycopene is susceptible to isomerize and isomerization may influence its bioactivity.In this paper,the authors studied the methodology of lycopene isomers detectation via COSMOSIL Cholester-HPLC system.The optimized conditions were as follows:a mixture of tetrahydrofuran and acetonitrile(10∶90,v/v) was used as mobile phase and its flow rate was 1mL/min,the injection volume was 20 μL,and the separating was monitored at 472nm.Confirmatory test was done and the result showed that this HPLC system was effective in lycopene isomers detection with ideal resolution and fairly good repeatability.