微生物酪氨酸解氨酶的研究进展

A Review of Tyrosine Ammonia Lyase in Microorganism

摘要:

微生物转化生产次生代谢物质是重要的方式,但已知的微生物中未发现次生代谢物质的前体物质肉桂酸-4-羟基化酶(C4H)和香豆酸,这限制了微生物转化生产一些次生代谢产物。酪氨酸解氨酶(TAL)能跳过C4H将L-酪氨酸(L-Tyr)转化为香豆酸,为此对微生物来源的TAL的酶学性质、晶体结构、催化机制及TAL基因工程方面的研究进行了阐述,发现了TAL研究中存在的问题并初步提出了解决思路。

Abstract:

Production of secondary metabolities by microbial transformation is a very effective means.However,no C4H and coumaric acid are found in microorganism,this limited the large scale of production of secondary metabolities from phenylalanine metabolism pathway.L-tyrosine was catalysised to form p-coumaric acid by tyrosine ammoni lyase without C4H,Base on the advantage,zymologic feature crystal structure,catalytic mechanism and genetic engineering of TAL were reviewed in this manuscript.