6种真菌发酵对马钱子降毒保效作用的初步研究

Pilot Study on Effects of Semen strychni Fermented by Six Different Fungi on Toxicity,Analgesia and Anti-Inflammation

摘要:

观察6种不同真菌发酵对马钱子毒性和镇痛、抗炎作用的影响。选择对马钱子药材适应性较强、菌丝生长旺盛的6种真菌菌种——裂褶菌、竹黄、单色云芝、鸡油菌、白僵菌和蝉花,在适生条件下对马钱子进行固态发酵,形成6种相应的马钱子发酵品——褶马菌质(SCSS)、竹马菌质(SBSS)、单马菌质(CUSS)、油马菌质(CCSS)、白马菌质(BBSS)和花马菌质(CGSS);以半数致死量(LD50)为指标,测定马钱子生品(RMSS)和炮制品(PSS)及6种不同发酵品经灌胃给药对小鼠的急性毒性;以双氯芬酸钠(DSE)和RMSS为阳性对照,观察6种不同发酵品对腹腔注射醋酸所致小鼠扭体反应的抑制(镇痛)作用以及对外涂巴豆油所致鼠耳壳肿胀的减轻(抗炎)作用。结果表明,马钱子通过6种真菌发酵后毒性降低,与炮制品基本一致;且它保持了马钱子原有的镇痛和抗炎作用,提示发酵可以为马钱子的降毒保效提供一条新途径。

Abstract:

The toxicity,the analgesic and anti-inflammatory effect of Semen strychni integrative fermentation with six different fungi,including Schizphylhls commne Fr.,Shiraia bambusocola P.Henn,Coriolus unicolor (L.:Fr.) Pat.,Cantharellus Cibarius Fr.,Beauveria bassiana (Bals.) Vuill and Cordyceps gunnii (Berk.) Berk were evaluated in this manuscript.When Semen strychni raw materials (RMSS) were solid-fermented with those fungi,the corresponding product were named as SCSS,SBSS,CUSS,CCSS,BBSS and CGSS,respectively.LD50 (i.g.) in mice were determined by the Bliss method to evaluate the acute toxicity of RMSS,processed Semen strychni (PSS) by traditional Chinese method and the six fermented products.The body writhing induced by i.p.0.1% acetic acid and the ear edema induced by 2% croton oil were used to test the analgesic and anti-inflammatory effects of six fermented Semen strychni products with the positive controls of diclofenac sodium (DSE) and RMSS.The toxicities of SCSS,SBSS,CUSS,CCSS,BBSS and CGSS were lower than that of RMSS,and were similar to that of PSS.The analgesic and anti-inflammatory effects of the aboved six fermented products were similar to that of RMSS.In conclusion the six different fermented Semen strychni had the same advantageous effects as that of PSS and the acute toxicity of them could be decreased compare to that of RMSS.