酵母转化茄尼醇生成辅酶Q10的超临界CO2体系条件

Supercritical CO2 Conditions of CoQ10 Biotransformation from Solanesol by Schizosaccharomyces prombe

摘要:

超临界CO2作为非水相介质对酵母转化茄尼醇生成辅酶Q10的底物和产物具有良好的溶解作用,但对微生物的存在会产生一定影响,选择合适的超临界CO2体系条件有利于辅酶Q10产量的提高。通过研究超临界CO2流体的压强、酵母细胞在超临界CO2流体中的反应时间、添加介质种类以及添加量等条件对粟酒裂殖酵母细胞活性和辅酶Q10产量的影响,确定对酵母细胞生存影响较小并显著促进辅酶Q10生产的超临界CO2体系条件为:超临界CO2压强为10.5 MPa,反应时间为16 h,向菌体中添加菌体湿重3倍的培养基,超临界CO2流体中辅酶Q10的产量达到55.71μg/mL。比液体深层发酵培养辅酶Q10产量提高97.5%。研究结果表明,通过优化酵母转化茄尼醇生成辅酶Q10的超临界CO2体系条件,可以显著提高酵母细胞生成辅酶Q10的能力。

Abstract:

Supercritical CO2(scCO2) as non-aqueous media could well dissolve substrates and products of coenzyme Q10(CoQ10) biotransformation reaction from solanesol,but it also had effects on microbial activity,so appropriate scCO2 conditions could improve CoQ10 yield.The effects of scCO2 on the catalytic activity of Schizosaccharomyces prombe(S.promb) and CoQ10 yield in different conditions were studied.The appropriate scCO2 conditions which could significantly promote the production of CoQ10 and could have little negative effects on the cells survival was: triple of cells’ wet weight of culture medium was added into the reaction system.,kept the pressure at 10.5 MPa for 16 h,the production of CoQ10 is 55.71 μg/mL,improved 97.5%.The results showed that optimization of scCO2 reaction system conditions could significantly improve the production of CoQ10.