超声波法提取1-脱氧野尻霉素

Study on Ultrasonic Extraction of 1-Deoxynojirimycin (DNJ) from Mulberry Leaves

摘要:

研究了利用超声波强化法从桑叶中提取1-脱氧野尻霉素(DNJ)。以乙醇溶液为浸提剂,超声波强化为辅助条件,提取了桑叶中的DNJ。考察了超声功率、料液配比、超声温度以及超声时间等因素对DNJ提取率的影响。结果表明:提取DNJ的最佳条件是超声功率125 W,每克桑叶浸提剂用量40 mL,超声温度70℃,超声时间20 min,DNJ的得率为0.091%,固形物中DNJ纯度为27.2%。

Abstract:

In this study,an ultrasonic-assisted extraction method was developed to extract 1-deoxynojirimycin(DNJ) from mulberry leaves and the process parameters of extraction were optimized by single factor experiment and orthogonal experiment.and acquired as follows: the ultrasonic power of 125 W,solid to water ratio of 1∶40,the extraction temperature of 70 ℃,and the extraction time of 20 min.with the optimum conditions,The yield and purity of DNJ was achieved at 0.091% and 27.2%.