MTG酶催化羊毛固定化溶菌酶及其抗菌性能

Immobilization of Lysozyme Catalyzed by MTG on the Wool and Antibacterial Action

摘要:

利用新型的生物催化剂微生物谷氨酰胺转胺酶(MTG)催化溶菌酶在羊毛织物上的固定化。通过SDS-PAGE方法研究了溶菌酶作为MTG催化底物的可行性,探讨了影响溶菌酶在羊毛上的固定化因素以及固定化后羊毛织物耐洗稳定性和抗菌性能。结果表明:羊毛织物在预处理后采用MTG催化可以实现溶菌酶的固定化,其中高锰酸钾预处理赋予了较好的固定化效果。实验得出的固定化条件为:溶菌酶用量2 g/L,固定化pH值7.0,固定化时间8 h,MTG用量20 U/g,在此条件下得到的固定化溶菌酶羊毛织物较吸附固定法具有更好的耐水洗稳定性及抗菌效果。

Abstract:

A new biocatalysts(MTG) was employed in this research to immobilize lysozyme on the wool fabrics.Based on the feasibility by SDS-PAGE which MTG was able to catalyze lysozyme,the research discussed the immobilizing conditions as well as the washing stability and the antibacterial action.The result was that lysozyme can be immobilized in the wool which was pretreated by chemical agents,particularly potassium permanganate.The immobilizing conditions defined by this research as follows: 2 g/L lysozyme,pH value 7.0,immobilizing for 8 h with the MTG 20 U/g.In these conditions the wool would obtain better washing stability and antibacterial action than that immobilized only by adsorption.