搅拌转速和通气量对灰树花深层发酵培养的影响

Effects of Agitation and Aeration on the Production of Mycelial Biomass and Exo-polysaccharides by Grifola frondosa GF9801

摘要:

采用15L搅拌式发酵罐研究灰树花的扩大培养条件，主要考察了搅拌转速和通气量对灰树花菌丝量、胞外多糖产率、溶氧、菌丝形态以及发酵液粘度的影响．研究结果表明搅拌转速和通气量能显著影响灰树花的生长，并且通过对灰树花菌丝球的大小、形态以及胞外多糖的分泌影响发酵液的粘度．并得出较为优化的培养条件为：温度25℃，通气量0．75vvm，搅拌转速80r／min，装液量60％；在此条件下，发酵10d后，灰树花菌丝体最大得率为22．95g／L，胞外多糖的得率为1．521g／L。

Abstract:

The effects of cultivation agitation intensity and aeration rate on the production of mycelial biomass and exo-polysaccharides(EPS) by Grifola frondosa GF9801 in a 15 L batch fermenter were investigated in this study.Results revealed that agitation intens