pH值对牛初乳中胰岛素样生因子-1(IGF-1)分离纯化的影响

Influence of Different pH on the Purification of Insulin-Like Growth Factor-1(IGF-1) from Bovine Colostrums

摘要:

通过搅拌式超滤器对牛初乳中的IGF-1超滤粗提，制备IGF-1的粗提物。对超滤条件的研究发现，在pH值为8．0碱性条件下的IGF-1回收率比在pH值为4．2酸性条件下高,将超滤后的粗提物分别用SDS-PAGE电泳检测，来验证每步骤的提纯效果,再将浓缩后的IGF-1粗提物用葡聚糖G-200柱层析进一步分离纯化，得到IGF-1纯品,每一步骤的IGF-1质量浓度利用放射免疫测定法（RIA）测定。

Abstract:

The primitive IGF-1 from colostrums was obtained by ultrafiltration, and a higher yields could be obtained at pH 8.0 other than pH 4.2. The rude IGF-1 after ultrafiltration was tested by SDS-PAGE, and the pure IGF-1 was obtained Chromatography with a Seph