解脂假丝酵母中神经酰胺合成的外部影响因素

The Factors Affecting the Ceramide Content in Yeast Yarrowia lipolitica

摘要:

为研究利用酵母发酵生产神经酰胺的可行性,利用高效液相色谱/蒸发光散射检测器作为分析手段,考察了解脂假丝酵母(Yarrowia lipolitica)在各种发酵条件下生产神经酰胺的能力.在各种可能的影响因素中,提高溶氧与乙醇刺激均能增加细胞内神经酰胺质量分数,在这两种条件下其占细胞干重的比例分别比对照提高2.5倍及3.3倍,最高可将其从占细胞干重的 0 25%提升到0 84%.其它培养条件对神经酰胺质量分数的影响则不明显或造成一定程度的下降.

Abstract:

Ceramide are widely distributed lipid molecules in eukaryotic cells, and it becomes important in cosmetic industry in recent years. To study the possibility of producing ceramide from yeast, the ceramide content in yeast Yarrowia lipolitica was detected u