麦胚凝集素红细胞凝集活性检测方法的改进

An Improved Protocol for Determination of Hemagglutinating Activity of Wheat Germ Agglutinin

摘要:

对红细胞进行蛋白酶处理、戊二醛固定,采用pH7.2等渗磷酸盐作为缓冲液,用光径0.5cm比色池测定凝集活性,读取测定终点的时间以2h为宜.建立的检测方法简单易行,得出的结果具有良好的灵敏度及重现性.

Abstract:

The procedure for determination of hemagglutination activity(HA) of wheat germ agglutinin(WGA) was improved. Major improvements include: 1). the self sedimentation of erythrocytes was deducted in the definition and calculation of HA; 2). erythrocytes were