BTH诱导水稻对稻瘟病系统获得抗性的mRNA差别显示分析

mRNA Differential Display of Systemic Acquired Resistance Induced by BTH in Rice against Blast Caused by Magnaporthe grisea

摘要:

运用mRNA差别显示技术对水稻感病品系G71受苯并噻二唑(Benzothiadiazole,BTH)诱导3d的应答反应进行了分析,从抗病及其相关基因保守结构域设计的10个引物组合的反应中获得了11个受BTH诱导的cDNA差异片段.反向Northern鉴定其中3个为阳性片段.Northern证实G71NBS14BI2的表达确实受BTH和稻瘟病菌的诱导.RFLP分析将G71NBS14BI2定位于水稻的第11条染色体上,且该片段座落于控制水稻穗瘟部分抗性的QTL区间.

Abstract:

The expression profiles a line G71 susceptible to isolate 92-183in response to Benzothiadiazole(BTH) were investigated using mRNA differentially display (DD). The leaves were sprayed with BTH for 3 daysat 4-leaf stage.A total of 11 differentially expresse