培养基及培养条件对普鲁兰酶的影响

Pullulanase from a New Isolated Strain

摘要:

从土壤中分离出一株产普鲁兰酶的菌株，初步鉴定为芽孢杆菌．通过优化发酵培养基及发酵培养条件，在２５０ｍＬ的摇瓶中可达到４．５μｍｏｌ／（ｍｉｎ·ｍＬ）的普鲁兰酶酶活．优化后的发酵培养基成分如下：玉米支链淀粉２ｇ／ｄＬ，蛋白胨２ｇ／ｄＬ，牛肉膏１．５ｇ／ｄＬ，ＮａＣｌ０．５ｇ／ｄＬ，ＭｎＳＯ４２μｍｏｌ／Ｌ．摇瓶发酵工艺条件：接种量１７％，温度３７℃，摇瓶转速２２０ｒ／ｍｉｎ，ｐＨ６．０，发酵周期３２ｈ．通过２Ｌ容积的发酵罐批式发酵，酶活可稳定在３．３μｍｏｌ／（ｍｉｎ·ｍＬ）左右

Abstract:

A bacteria producing pullulanase was isolated from soil with a special method and identified to be Bacillus sp. on the basis of its morphological and physiological characteristics, referring to "Bergey's Mannual of Determinative Bacteriology(8