磷脂酶D催化反应中的有机溶剂效应

The Solvent Effect on Phospholipase D Catalysis

摘要:

对酶催化反应中的有机溶剂效应进行了深入讨论，引用溶剂化概念解释了有机溶剂对反应平衡状态，反应速率和反应选择性的影响。此外，考察了２４种有机溶剂对磷脂酶Ｄ催化反应的影响，提出了有机溶剂作用机理，即有机溶剂使酶蛋白微粒和底物磷脂表面更具疏水性，从而增加了酶和底物的相互吸引。主题词

Abstract:

The effect of chemical equilibrium,reaction rate and selection caused by organicsolvents in enzymatic catalysis were discussed with the concept of solvation, The rule ofsolvation on phospholipase D catalysis was found through testing 24 kinds of organic s