直接发酵木糖为酒精的新酵母基因工程菌的选育

The Selection of new Yeast Species Fermenting Directly Xylose to Ethanol by Cloning of Isomerase Gene

摘要:

裂殖酵母,Schizosacchatomyces porebe不能发酵木糖,也不能用它来增殖细胞。但是,这种酵母能发酵木酮糖为酒精,产率和转化率都很高。这表明,它缺少异构酶基因,如果将其它微生物的这种基因克隆到酵母中去,它就能直接发酵木糖。本文介绍了Schizosaceh.porebe的五碳糖磷酸代谢途径,这种代谢途径使得它成为用于这一目的的理想菌株。含有异构酶基因的DNA片段来自Clark和Carbon的大肠杆菌基因库,它的大小为2.4kb,PstⅠ-抗性。将这个基因克隆到上述酵母中去,得到的酵母基因工程菌株不仅能利用木糖作为唯一碳源和能源,还能发酵木糖为酒精,它具有在工业上应用的潜力

Abstract:

Fission yeast, schizosaccharomyces pombe, can not be used for fermenting xylose sugar and for cell growth. However, the yeast can be used to ferment xylulose, an isomerized product of xylose, to ethanol with high yield and efficiency. This shows that a xy