WU Wei , TAN Long fei , YANG Lian sheng
2002, 21(2).
Abstract:Cinnaldehyde was microencapsulated with the method of spray drying using the gum arabic and maltodextrin as the principal wall materials.The effects of processing conditions, such as composition of wall material,dosage of emulsifier,wall material concentr
SU Chang , XIA Wen shui , YAO Hui yuan
2002, 21(2).
Abstract:The effects of reaction temperature, pH, time and enzyme concentration on the hydrolysis of chitosan by papain were investigated. The relationship between hydrolysis and structure of chitosan (deacetylation degree and molecular weight) by papain was
XIE Li ping , ZHUGE Jian , WANG Zheng xiang
2002, 21(2).
Abstract:采用PCR技术扩增了运动发酵单孢菌Zymomonasmobilis的两个产乙醇的关键酶基因pdc和adhⅡ ,分别克隆到载体 pUC19和pUC18中 ,形成重组质粒 pUC19::pdc和 pUC18::adh ,从中分离出两基因并串联到经EcoRⅠ酶切的 pUC18中 ,转化大肠杆菌E .coliDH5α获得重组质粒 pPA .携带pPA的重组菌Pp +a被证明具有丙酮酸脱羧酶酶活且提高了乙醇脱氢酶酶活 .通过代谢工程在Escherichiacoli中成功地构建了乙醇合成途径
WANG Yuan feng , ZHANG Min , ZHENG Jian
2002, 21(2).
Abstract:The processing of green turbid vegetable juice of artemisia selengensis leaves was studied in this paper. The beverage with brilliant colour,rich nutrition and good taste was produced by color protecting,squeezing,centrifugation, flavoring, homogenizatio
HUANG Hai , YANG Rui jin , WANG Zhang
2002, 21(2).
Abstract:The effect of 10 types of activated carbon and 6 types of anion exchange resin on decolorizing of xylo oligosaccharides (XOS) syrup, and the colorant sorption isotherms were investigated. Results showed that activated carbons AC 2 and AC 6 and the anio
PAN Jun hua , PAN Zhong ming , ZENG Mei juan , ZHANG Xing yuan
2002, 21(2).
Abstract:通过正交试验探讨了碳源、氮源及豆饼水解液对赖氨酸产量的影响 ,确定了最佳工艺条件 .添加醋酸盐、生物素、泛酸盐等对FP0 94菌株中由磷酸烯醇式丙酮酸、丙酮酸和草酰乙酸构成的“三角区”进行扰动 ,定性分析了此扰动对赖氨酸合成所需前体物代谢流量的调节机制 .添加2 .0 %的醋酸钙可使赖氨酸的产量及葡萄糖的转化率均得到显著提高 ,生物素的添加量在 2 0 0~5 0 0 μg/L时可明显提高赖氨酸的产量和转化率 .泛酸钙的限量添加可弱化泛酸缺陷突变株中丙酮酸向乙酰辅酶A的转化 ,并提高天冬氨酸 β 半醛流量向赖氨酸合成分支的分配 .
ZENG Mei juan , ZHANG Ke chang
2002, 21(2).
Abstract:结合工业发酵的特点 ,研究了营养因子对鸡腿蘑 (毛头鬼伞 )胞外多糖产量的影响 ,筛选到适合其胞外多糖分泌的优化培养基配方 :葡萄糖 2 .0 % ,玉米粉 (6 0目 ) 1.0 % ,麸皮粉 (6 0目 )0 .3% ,VB110mg/L ,KH2 PO4 0 .1% ,MgSO4 ·7H2 O 0 .1% ,在此基础上进行摇瓶发酵曲线的测定 ,确定生产胞外多糖的适宜发酵周期为 4d ,发酵液中产量最高可达 96 .3mg/dL .
CHEN De wei , WANG Liang , ZHANG Min
2002, 21(2).
Abstract:研究了新仓小茄子中的果胶酯酶和多酚氧化酶的最适温度、pH、热稳定性以及钙离子和氯化钠对酶性质的影响 .利用茄子中酶的性质 ,在保证色泽和风味的前提下 ,激活果胶酯酶以改善热烫杀青后茄子的质构 ,提高其硬度 .实验表明 ,采用含 0 .2 4 4mol/L的NaCl、pH 9.2、0 .1mol/L的碳酸钠 碳酸氢钠缓冲液 ,于 5 2 .6℃下低温浸泡 18.9min后再热烫杀青 ,可以较好地提高茄子的质构 .
2002, 21(2).
Abstract:研究了不同的提取温度、提取时间和加水比 ,对姬松茸液体发酵菌丝体和固体栽培子实体粗多糖提取得率的影响 .采用二元二次回归的分析方法得姬松茸菌丝体和子实体的最佳提取条件为 :菌丝体为 10倍加水量于 90℃提取 3.3h ;子实体为 10倍加水量于 90℃提取 3.4h .提取率可以分别达到 1.376 %和 1.6 40 % .在此基础上 ,比较了乙醇质量分数和提取液 pH对姬松茸子实体和菌丝体多糖沉淀特性的影响 .
DUAN Zhen hua , ZHANG Min , HUAN Yan jun , TAO Qian , YU Shui bao
2002, 21(2).
Abstract:研究了半流质高能食品的制取工艺 .用蛋白酶降解大豆蛋白和猪肉蛋白 ,比较了生肉和熟肉分别作为酶解底物的差异 ,发现使用熟肉为底物效果较好 ;使用AR10 0 0型流变仪测定样品的粘度 ,并借助SAS软件对影响样品粘度的因素进行了回归分析 ,发现淀粉和油的用量对粘度影响显著 ;结合感官评价检验 ,制得甜、辣和咸 3种不同口味的半流质高能食品 ,它们在 2 5℃时的粘度为0 .2 135~ 0 .30 6 9Pa·s ,能量密度大于 13.8kJ/ g .
ZHANG Min , ZHANG Xue mei , XIAO Gong nian , ZHANG Tai wei , WANG Yuan feng
2002, 21(2).
Abstract:测试了杜瓜子的基本组成成分 ,其中蛋白质和脂肪质量分数较高 ,达 32 .2 4 %和 5 2 .33% ;得出了杜瓜子的较佳脱苦条件为 10 g/dL食盐水煮 15min ,同时对杜瓜子的加工工艺进行了优化 ,得出的最佳干燥参数为 :烘温 10 0℃ ,1.5cm堆积厚度 ,烘时 2 .5h ,隔 30min翻动一次 .
2002, 21(2).
Abstract:采用可食性膜对樱桃番茄进行保鲜实验 ,测定在贮藏过程中樱桃番茄中的总糖、总酸、VC含量的变化以及其呼吸强度、失重率、烂果率和质构的变化 ,可以看出涂膜保鲜的效果良好 ,樱桃番茄的货架期在 (2 8± 3)℃条件下达到 15d .
2002, 21(2).
Abstract:The efficiency of releasing of microencapsulated carnitine in the stimulated gastric and intestinal fluids was studied. The kinetics of the microencapsulated carnitine in the simulated intestinal fluids was also investigated. The release mechanism of micr
2002, 21(2).
Abstract:Grifola frondosa is a kind of valuable edible and medical fungus.In this article, we inoculated solid medium by means of stirring inoculation. Growth of mycelium was accelerated greatly and cultivation period was reduced greatly by this cultivation.This
2002, 21(2).
Abstract:The immunomodulatory function of polysaccharides isolated from Agaricus blazei produced by submerged fermentation was studied. The results showed that the proliferation function of immunological organs was promoted, the genesis of delayed type hyperse
JIANG Yuan rong , QIAN Hai feng , MEN De qi
2002, 21(2).
Abstract:研究了纳他霉素 (Natamycin)与乳酸链球菌素 (Nisin)在传统酿造酱油中的防霉应用情况 ,以及不同浓度纳他霉素与乳酸链球菌素的复合使用情况 .结果表明 :纳他霉素防止传统酿造酱油霉变的最小抑菌浓度 (MIC)为 2 0 μg/kg ,而通过与乳酸链球菌素复合 ,可以有效降低MIC至 8μg/kg ,符合食品添加剂的卫生要求 .
2002, 21(2).
Abstract:In order to investigate the effect of pumpkin polysaccharide (PP) on normal mice and alloxan induced diabetic mice, the experiment adopted a kind of new technology to extract PP from pumpkin powder. This crude polysaccharide was fractionated by ion exc
SONG Jun mei , TAO Guan jun , TANG Jian , DING Xiao lin
2002, 21(2).
Abstract:The C 21 steroidal aglycones in Baishouwu were isolated and characterized. The C 21 steroidal glycosides extracted in advance from Baishouwu were acidolyzed and the general aglycones were extracted. Two kinds of aglycones were obtained after chr
ZHANG Liang , SHI Gui yang , DONG Shi jian
2002, 21(2).
Abstract:在生物转化法生产麻黄碱研究过程中 ,建立了一种检测转化液中麻黄碱含量的方法———薄板层析双波长扫描法 .该方法以硅胶GF2 54 为吸附剂 ,以甲醇 浓氨水混合液 (V(甲醇 )∶V (浓氨水 ) =98∶2 )为展开剂 ,0 .2 %茚三酮丙酮溶液为显色剂 ,扫描波长λS=5 10nm ,λR=70 0nm .并运用该法考察了 4株初筛菌株的转化能力 .
GUO Shi dong , LI Hua , WANG Hui bing , XIONG Yi qiang
2002, 21(2).
Abstract:通过工厂中试发现 ,入模水分是影响大豆皮制粒产量和生产电耗的重要因素 .采用调质前预加水的方法 ,可将制粒产量从 2t/h提高到 8t/h ,电耗由 5 2kW·h/t降低到 13kW·h/t.实验所得大豆皮颗粒的容重达到 6 46~ 710 g/L .大豆皮颗粒形状稳定 ,坚实度大于 (等于 ) 94 % .首次采用制粒法杀灭大豆或大豆制品中的脲酶 ,当制粒温度达 98℃时 ,脲酶杀灭率可达 6 2 %~ 6 4% ,制粒后保持颗粒出模温度 4 0min ,脲酶杀灭率由 6 2 %~ 6 4%增加至 76 %~ 77% .
LIU Jian wen , LE Guo wei , SHI Yong hui , WANG Zhi jun , WANG Yuan
2002, 21(2).
Abstract:动物生长抑制激素 (Somatostatin ,SS)基因克隆至含有乙肝表面抗原的 pcDNA3s质粒载体上 ,生长抑素基因插入到乙肝表面抗原的 5′末端 ,构建生长抑素DNA疫苗 .经PCR反应扩增出含有SS和HBsAg的融合基因片段 ,并经测序证实SS基因已成功重组到 pcDNA3s质粒上
XU Chuan lai , LE Guo wei , YAO Hui yuan
2002, 21(2).
Abstract:Through the experiments, it was proved that PST liposome prepared by reverse phase evaporation could be in the most stable state under neutral pH of environmental solution and refrigerated storage. Encapsulation was 89%. This is the first step of the re
ZHU Jian jin , ZHU Chun lei , SHE Xiao hui
2002, 21(2).
Abstract:A Chinese traditional medicine composed of A. membranaceus Bge, C. tangshen Oliver, Angelica sinensis, Ligustrum lucidum Ait.,Massa Medicata Fermentata, A. chinensis Koidz., Psoralea corylifolia L., Leonurus heterophyllus Sweet. Experiments were cond
CHEN Guo qiang , ZHANG Guang , ZHAO Kai , TIAN Ge , CHEN Jin chun , WU Qiong
2002, 21(2).
Abstract:Polyhydroxyalkanoates (PHA),a microbially synthesized polyesters, have attracted increasing attentions from scientific and industrial communities for their combination of several properties including biodegradability, biocompatibility and optical activit
ZHUGE Jian , ZHAO Zhen feng , FANG hui ying
2002, 21(2).
Abstract:In this article, the relation ship between structure and function of polysaccharides is summarized according to different activities. For the polysaccharides with immunity enhancement activity, the activity is affected by the constitutes of backbone cha
2002, 21(2).
Abstract:This paper mainly discusses the principle, development and effect elements of affinity filtration (including the choices of escorts, ligands and membranes). and its application in bioengineering for the separation of enzymes, proteins and other bioactive
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