Abstract:[Objective]This study constructed a stable lipopeptide (LP) delivery system to improve the stability and dispersibility of LP, thereby enhancing their antibacterial efficacy.[Method]LP particles were constructed with chitosan (CS) and sodium alginate (SA). Assembly property changes were evaluated by means of different construction and single factor experiments. Interactions among CS, LP, and SA were investigated by Fourier transform-infrared spectroscopy (FT-IR), and the rheological properties of the system were examined by a rheometer. Antibacterial activity of LP particles was evaluated with Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) as model microorganisms.[Result]LP particles prepared by dripping CS with the relative molecular mass of 300 000 into a mixed solution of SA (low viscosity) and LP at the CS∶LP∶SA mass ratio of 1∶1∶1, 150 r/min, and pH 4 achieved the best encapsulation performance. The LP particles prepared under the conditions above showed the encapsulation rate of (92.95±1.31)%, loading content of (41.14±1.63)%, particle size of 420~489 nm, and Zeta potential of (–21.56±3.54) mV. FT-IR results indicated that LP was encapsulated inside the particles, with SA forming the outer shell. Rheological data confirmed a hydrogel-like structure. In addition, LP particles showed strong inhibitory effects against both E. coli and S. aureus and resistance to thermal and UV stress.[Conclusion]LP particles constructed in this study not only reduce LP aggregation but also provides effective protection against environmental stress, offering a promising strategy for the delivery of LP-based antimicrobials in fields such as food preservation.
