Abstract:[Objective] This study aimed to explore the ameliorating effects of hawthorn water extract on aging-related behaviors in wild-type Drosophila melanogaster, the model organism. [Methods] Drosophila melanogaster individuals were allocated into two groups: a control group (basal medium) and an experimental group (basal medium + hawthorn water extract). The lifespan, vertical climbing ability, reproductive ability, heat stress resistance, and malondialdehyde (MDA) molality of Drosophila melanogaster were measured to analyze the anti-aging ability of hawthorn water extract. [Result] Compared with the control group, the experimental group showed a 25.06% increase in lifespan in males, enhanced vertical climbing ability and heat stress resistance in both genders (P<0.05), and a decrease in MDA molality (P<0.05). Moreover, the hawthorn water extract effectively prolonged the lifespan and delayed aging in Drosophila melanogaster without significantly compromising reproductive ability. [Conclusion] The hawthorn water extract can prolong the lifespan and delay the aging of Drosophila melanogaster, with low reproductive toxicity. This study lays a foundation for deciphering its anti-aging mechanism and provides a reference for the development and application of hawthorn water extract in health foods.
