Anti-fatigue Effects of Olive Pomace Extracts in Mice

Abstract:

[Objective] The study aimed to evaluate the anti-fatigue effects of olive pomace (OP) extracts in mice. [Method] Phenolic compounds were enriched from the pomace of five olive varieties and quantified and analyzed via high-performance liquid chromatography (HPLC). The anti-fatigue effects of OP extracts in mice were systematically assessed through exhaustive swimming, forelimb grip strength, and biochemical indicator. [Result] The OP extract of ‘Gordal’ had the highest total phenol mass fraction and antioxidant activity. The animal test results demonstrated that OP extracts significantly prolonged exhaustive swimming time, enhanced forelimb grip strength, and reduced serum ammonia and blood glucose levels while increasing blood urea nitrogen (BUN) and serum creatine kinase (CK) levels, suggesting its potential to improve muscle recovery after exercise and alleviate exercise-induced metabolic stress. Furthermore, histopathological examinations revealed no organ damage caused by OP extract supplementation. [Conclusion] This study not only provides scientific evidence for the high-value-added utilization of OP but also offers new technical insights into the development of natural safe supplements with anti-fatigue effects.