Enhancement of Xylose Metabolism and Bacterial Cellulose Biosynthesis in Komagataeibacter xylinus Through Multiple Mutagenesis Techniques

Abstract:

[Objective] This study aimed to address the low xylose metabolic efficiency and limited bacterial cellulose (BC) biosynthesis capacity of Komagataeibacter xylinus (K. xylinus) by exploring the use of multiple physical mutagenesis techniques to enhance xylose utilization and BC biosynthesis. [Method]Ultraviolet (UV) mutagenesis, atmospheric and room temperature plasma (ARTP) mutagenesis, and UV-ARTP combined mutagenesis were employed to improve K. xylinus. Pure xylose and mixed carbon-source fermentation systems were established to evaluate the fermentation performance of the mutant strains. Efficient and genetically stable mutants were screened by comparing BC yield and xylose consumption. [Result] The ARTP-mutagenized strain ARTP-Mix-1 achieved a BC yield of 5.07 g/L and xylose consumption of 4.06 g/L, which represented increases of 73.04% and 13.50 folds compared with those of the wild-type strain WT-Mix, respectively, and both values were higher than those of the UV-mutagenized strains. Furthermore, the mutant strain CM-Mix-20 produced by UV-ARTP achieved a BC yield of 6.00 g/L and xylose consumption of 4.70 g/L, which represented increases of 104.78% and 15.79 folds compared with those of WT-Mix, respectively. [Conclusion] This study provides a new strategy for the industrial production of BC and the efficient utilization of xylose resources.