Improvement of Glucose and Lipid Metabolism by an Anthocyanin-dietary Fiber Drink Based on Modulation of the Gut Microbiota

Abstract:

[Objective] This study aims to investigate the mechanisms by which an anthocyanin-dietary fiber drink ameliorates glucose and lipid metabolism disorders induced by a high-sugar, high-fat diet through modulation of the gut microbiota. [Methods] In vitro experiments were conducted to measure the α-glucosidase inhibition, lipase inhibition, and cholesterol clearance rates. The 16S rDNA sequencing was employed to assess structural changes in the fecal microbiota of the mice fed with a high-sugar, high-fat diet following anthocyanin-dietary fiber drink intervention. The levels of lipopolysaccharides (LPS), total bile acids (TBA), and short-chain fatty acids (SCFAs) were determined by ELISA and GC-MS. [Results] Anthocyanin-dietary fiber drink significantly increased the α-glucosidase inhibition, lipase inhibition, and cholesterol clearance rates (P<0.05), and it outperformed anthocyanin or dietary fiber used alone (P<0.05). Furthermore, anthocyanin-dietary fiber drink improved the gut microbiota structure, elevated the levels of SCFAs and TBA, and reduced the levels of LPS in the mice fed with a high-sugar, high-fat diet. [Conclusion] Anthocyanin-dietary fiber drink synergistically modulates the structure and metabolism of the gut microbiota and its efficacy exceeds that of individual components. This study provides a theoretical foundation for the development of functional drinks.