Research Progress of CRISPR/Cas Systems in Detection of Bacterial Pathogens

Abstract:

Bacterial infectious diseases have long posed a serious challenge to global public health. Although conventional pathogen detection methods have made some achievements in clinical application, they still face the limitations of low sensitivity, long time consumption and so on. As new gene editing tools, CRISPR/Cas systems are widely used in pathogen detection because of the high efficiency and accuracy. The author summarized the technical principle, advantages, and disadvantages of CRISPR/Cas systems, and briefly reviewed the research progress of using CRISPR/Cas technology to detect different bacterial genera in recent years, aiming to provide reference for the detection of bacterial pathogens in the future.