Abstract:[Objective]To explore the application prospects of Citri Reticulatae Pericarpium in the food industry and efficiently extract the active component hesperidin, while developing an effective purification process to enhance the purity and functional value of the active ingredients.[Method]Ultrasonic-microwave assisted extraction (U-MAE) was employed to extract the hesperidin from Citri Reticulatae Pericarpium, followed by a three-stage purification system guided by dissolution characteristics involving alcohol removal by concentration, gradient ethanol washing, and pH adjustment to purify hesperidin. Single factor experiments and response surface methodology were used to optimize the extraction parameters. The antioxidant activity and α-glucosidase inhibitory activity of the final product were also evaluated.[Result]Under the optimal extraction conditions (60% ethanol-water solution by volume, microwave power of 220 W, extraction time of 3.5 min, and material/liquid ratio of 1 g∶45 mL), the hesperidin yield reached (43.3±0.2) mg/g, demonstrating significant efficiency advantages over traditional extraction methods. After three-stage purification, the mass fraction of hesperidin increased from (41.70±0.07)% to (96.09±0.88)%. The final product demonstrated excellent antioxidant activity (ABTS free radical scavenging IC50 of 135.57 μg/mL) and α-glucosidase inhibitory activity (IC50 of 102.13 μg/mL).[Conclusion]The U-MAE and three-stage purification system developed in this study enables efficient extraction and purification of active components from Citri Reticulatae Pericarpium, significantly enhancing their purity and functional values. This provides theoretical basis and technical support for the application of Citri Reticulatae Pericarpium in the development of functional foods and offers new ideas for the development and utilization of other traditional Chinese medicinal materials that are both food and medicine.
