Mechanisms of Antioxidant and Anti-aging Activities of Asini Corii Colla Cake

Abstract:

Objective This study aims to decipher the mechanisms underpinning the antioxidant and anti-aging activities of Asini Corii Colla cake prepared with Asini corii colla, walnut kernel, black sesame, and rice wine.Method In the animal experiments, 50 SPF-grade male ICR mice were randomly assigned into 5 groups (n=10): blank negative control, model positive control, and low-dose (1.67 g/kg BW), medium-dose (3.33 g/kg BW), and high-dose (6.67 g/kg BW) Asini Corii Colla cake. After 30 of continuous feeding, the serum levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), lipid peroxidation product malondialdehyde (MDA), and protein carbonyl (PCO) were determined for each group of animals. In the human trial, 110 subjects were randomly allocated into experimental and control groups. The experimental group was given Asini Corii Colla cake, and the control group had no intervention. The intervention period was 3 months. The serum levels of SOD, GSH-Px, and MDA as well as skin moisture and oil content were measured, and the VISIA test was carried out.Result In the animal experiments, Asini Corii Colla cake groups showed significantly declined serum levels of MDA and PCO and significantly elevated serum levels of SOD and GSH. In the human trial, the experimental group showed significantly increased serum levels of SOD and GSH-Px compared with the control group, indicating that Asini Corii Colla cake contributed to antioxidant effects. Meanwhile, the results of human facial index test showed that the consumption of Asini Corii Colla cake increased the skin moisture content and the facial skin oil secretion, which could ameliorate facial aging, spots, and UV-induced pigmentation to a certain extent, suggesting that the consumption of Asini Corii Colla cake could help the skin to resist aging.Conclusion This study provides a basis for investigating the mechanisms of the antioxidant and anti-aging activities produced by Asini Corii Colla cake.